A fluorescent detector is a device used to detect and measure fluorescent signals that occur when light interacts with certain substances. These detectors are widely used in various fields, including chemistry, biology, medicine, and environmental science.
Main characteristics of fluorescent detectors:
Working principle:Fluorescent detectors operate based on the phenomenon of fluorescence, where molecules absorb light of a specific wavelength and emit it as light of a different wavelength.
Types of fluorescent detectors:
Manual: Allow the user to adjust parameters for various experiments.
Automated: Optimized for high-throughput applications, capable of automatically performing sample analysis.
Application:
DNA and RNA analysis: Used in molecular biology to detect and quantify nucleic acids.
Chemical analysis: Used to identify and quantitatively measure various chemical compounds.
Environmental studies: Used for monitoring pollution and studying ecosystems.
Advantages: High sensitivity and specificity.Ability to measure small amounts of substances.Rapid data processing.
Disadvantages: May require complex sample preparation.Sensitive to environmental conditions such as temperature and pH.
Conclusion
Fluorescent detectors are powerful tools for research and analysis, enabling accurate data collection on various chemical and biological substances. Their use requires an understanding of the principles of fluorescence and the specifics of working with each type of detector.
The fluorescence detector is designed for highly sensitive and selective detection of compounds that exhibit intrinsic luminescence. These include polycyclic aromatic hydrocarbons (PAHs), mycotoxins, carbamates, vitamins, amino acid derivatives, and others. The fluorescence detector enables trace analysis even in complex samples. Two independent monochromators ensure the highest reproducibility of excitation and emission wavelengths, which facilitates the adjustment of methods to optimal detection conditions for individual components.
Specifications of FLD-3100 and FLD-3400 Fluorescence Detectors
- Wavelength range: excitation - 200-630 nm (optionally up to 880 nm), emission - 220-650 nm (optionally up to 900 nm)
- Wavelength accuracy: ±2 nmWavelength reproducibility: ±0.2 nm
- Data collection rate: up to 200 Hz
- Number of simultaneously recorded signals: up to 4 at different wavelengths (FLD-3400)
- Sensitivity: Signal-to-Noise ratio >2100 for the water Raman line
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